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Goat Anti-HPK1 / MAP4K1 Antibody
Peptide-affinity purified goat antibody
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q92918 |
| Other Accession | NP_009112, 11184 |
| Reactivity | Human |
| Predicted | Mouse, Rat, Pig, Dog |
| Host | Goat |
| Clonality | Polyclonal |
| Concentration | 100ug/200ul |
| Isotype | IgG |
| Calculated MW | 91296 Da |
| Gene ID | 11184 |
|---|---|
| Other Names | Mitogen-activated protein kinase kinase kinase kinase 1, 2.7.11.1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1, MAP4K1, HPK1 |
| Dilution | WB~~1:1000 E~~N/A |
| Format | 0.5 mg IgG/ml in Tris saline (20mM Tris pH7.3, 150mM NaCl), 0.02% sodium azide, with 0.5% bovine serum albumin |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Goat Anti-HPK1 / MAP4K1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MAP4K1 (HGNC:6863) |
|---|---|
| Synonyms | HPK1 |
| Function | Serine/threonine-protein kinase, which plays a role in the response to environmental stress (PubMed:24362026). Appears to act upstream of the JUN N-terminal pathway (PubMed:8824585). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed:26437443). May play a role in hematopoietic lineage decisions and growth regulation (PubMed:24362026, PubMed:8824585). Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (By similarity). |
| Tissue Location | Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine |
Provided below are standard protocols that you may find useful for product applications.
References
Phosphorylation of CARMA1 by HPK1 is critical for NF-kappaB activation in T cells. Brenner D, et al. Proc Natl Acad Sci U S A, 2009 Aug 25. PMID 19706536.
Proteasome-mediated degradation and functions of hematopoietic progenitor kinase 1 in pancreatic cancer. Wang H, et al. Cancer Res, 2009 Feb 1. PMID 19141650.
Prostaglandin E2 activates HPK1 kinase activity via a PKA-dependent pathway. Sawasdikosol S, et al. J Biol Chem, 2007 Nov 30. PMID 17895239.
Caspase-cleaved HPK1 induces CD95L-independent activation-induced cell death in T and B lymphocytes. Brenner D, et al. Blood, 2007 Dec 1. PMID 17712048.
Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening. Wu C, et al. Proteomics, 2007 Jun. PMID 17474147.
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